The ability to retrieve the results and work with both them and the raw data is critical our experience points to the importance of bioinformatics tools that will allow us to examine gating robustness, combine manual and automated gating, and perform exploratory data analysis. This is easily accomplished in commercial flow cytometry packages but it is difficult to work computationally with the results of this process. In flow cytometry, different cell types are usually selected or “gated” by a series of 1- or 2-dimensional geometric subsets of the measurements made on each cell.
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